Paolo Pinton Lab__Signal Transduction
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The technology of Extracellular Flux analyzer, introduced by Seahorse Bioscience, is based on calibrated plates with fluorescent compounds for the simultaneous measurement of oxygen levels and pH in the medium. Through kit provided by the manufacturer can be performed in situ normalization to derive the activity of glycolysis and oxidative phosphorylation in intact cells. Also through other tool kit can provide even more detailed information on health status as well as mitochondrial fatty acid oxidation. Finally, as alternative, cell methabolism can be assessed by customized assay. |
