Paolo Pinton Lab__Signal Transduction

Inverted confocal laser scanning microscope for the observation of fixed or live samples with high accuracy in XYZ. Acquisition depth to 500 nm

Lens

• 10X Plan-Neo / 0.3 NA
• 40X Plan-Neo / 1.3 NA Oil
• Plan-Apo 63X / 1.4 NA Oil

Laser lines
• Adjustable Argon 458/477/488 / 514nm at 30mW
• HeNe 543nm at 1 mW
• HeNe 633 nm at 3 mW

Detector
• 3 PMTs (photomultiplier tubes)

Fluorophores observable
• ECFP, Cerulean, AlexaFluor 430;
• FITC, Cy2, AlexaFluor 488, EGFP;
• EYFP, Venus, AlexaFluor 514;
• Rhodamine, Cy3, mCherry, RFP, R-PE;
• Cy5, APC, AlexaFluor 647;

Applications
• Z sectioning and 3D reconstruction,
• Co-location of more protein and / or dyes
• FRET

Software
Zeiss Zen Blue

A. Confocal image of HeLa cells stained with FM4-64 to the plasma membrane (Red) and Lysotracker Green to lysosomes (Green)

B. Serial sections of U2OS cells nucleus expressing the recombinant protein H2B-RFP for histones staining

C. Confocal image of HeLa cells expressing the mitochondrial marker Mitotracker Deep Red (blue), the endoplasmic reticulum marker Sec61B-GFP (green) and the lysosomal dye Lysotracker Red (red)