PhD in Biomedical Sciences and Biotechnology

 

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CURRICULUM VITAE FATEMEH MOHAGHEGHI


Date and place of birth: 21/03/1985, Tehran

Nationality: Iranian

Affiliation and official address: Neuroscience Research Center of Shahid Beheshti University of Medical Sciences, Tehran, Iran


Telephone: 00982122429765
e-mail: f_mhghgh@yahoo.com

 

EDUCATION

2003-2007 B.Sc. in Biology  (University of Shahid Beheshti)

2007-2010 M.Sc. in Physiology (University of Shahid Beheshti)

2014-2017 Ph.D. in Biomedicine and Biotechnology (University of Ferrara)


CAREER/EMPLOYMENT
2010-2012 Research assistant (University of Shahid Beheshti)

2012-2014 PhD student in ICGEB, Trieste, Italy

From  2017 to present, Research assistant (University of Shahid Beheshti)

 

RESEARCH EXPERIENCES
1. Animal stroke model: Induction of focal cerebral ischemia in rat: middle cerebral artery occlusion (MCAO)

2. Animal stroke model:  Induction of global cerebral ischemia in rat:  4 vessels occlusion (4VO)

3. Animal handling, rat stereotaxic surgery and EEG recording

4. Cell culture: Good experience in manipulating different human and mouse cell lines

5. Gene manipulation: gene knockdown, gene overexpression, mutagenesis, and transfection

6. Cloning: minigene construct manipulation and mutagenesis

7. Protein-RNA interaction: Pulldown assay

8. Protein-RNA interaction: RNA- immunoprecipitation (RIP)

9. Protein-protein interaction: Co-immunoprecipitation (Co-IP)

10. Western blotting

11. Oligonucleotides designing for gene amplification (Primers), gene Knockdown (siRNA and shRNA), and for mutagenesis.

12. in vitro RNA transcription

13. RNA extraction

14. RT-PCR

15. Real-time PCR

16. Basic experience in immunohistochemistry, immunocytochemistry, microscopy, and ELISA

 

AWARDS
2012, PhD fellowship from International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

 

Researcher unique identifiers: ORCID: 0000-0002-0757-394X